Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Article Title: Enhancing Tendon Regeneration: Investigating the Impact of Topography on the Secretome of Adipose-Derived Stem Cells.
doi: 10.1002/advs.202417447
Figure Lengend Snippet: Figure 10. ACM improves cell proliferation and tendon differentiation of TSPCs by activating MAPK and Integrin pathways. A) Western blot of p-MEK and MEK in TSPCs after treatment of RCM, RCM+U0126, ACM, or ACM+U0126 for 18 h. B) Western blot of p-ERK, ERK in TSPCs after treatment of RCM, RCM+U0126, ACM, or ACM+U0126 treatment for 18 h. C) Gene expression of PCNA in different groups at day 3. D) Proliferation of TSPCs measured by CCK-8 at 1, 3, and 5 days, n = 5 technically independent samples for each group, **p < 0.01 ****p < 0.0001. E) Gene expression of SCX, TNMD, and MKX in different groups at day 3. Levels at the RCM group were set as 1. Data were shown as Mean ± SD, n = 3 technically independent samples for each group, *p < 0.05, **p < 0.01. F) Gene expression of PCNA in different groups at day 3, ***p < 0.001. G) Proliferation of TSPCs measured by CCK-8 at 1, 3, and 5 days, n = 5 technically independent samples for each group, ****p < 0.0001. H) Gene expression of SCX, TNMD, and MKX in different groups at day 3. Levels at the RCM group were set as 1. Data were shown as Mean ± SD, n = 3 technically independent samples for each group, *p < 0.05.
Article Snippet: After the membranes were blocked in QuickBlock Blocking Buffer (Beyotime, P0252) for 15 min, the primary antibodies against p-MEK (1:1000, CST, 9154), MEK (1:1000, CST, 8727), p-ERK (1:2000, CST, 4370), ERK (1:1000, CST, 4695), β-arrestin (1:1000, CST, 30036), and GAPDH (1:200000, Proteintech, 60004-1-lg) were used to incubate the membranes.
Techniques: Western Blot, Gene Expression, CCK-8 Assay
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